[ARVD-FORUM] ARVD and Brugada Syndrome. Dr. Perez Riera

ARVD Symposium info at arvd-symposium.org
Tue Apr 12 22:22:30 ART 2005


English - Portuguese

In 1996, Corrado et al (Corrado D, Nava A, Buja G, et al. Familial 
cardiomyopathy underlies syndrome of right bundle branch block, ST segment 
elevation and sudden death. J Am Coll Cardiol 1996, 27: 443-448) presented 
data in support of the hypothesis that a subpopulation of patients with 
ARVC/D, referred to as "concealed forms," present with the typical clinical 
and electrocardiographic features of the Brugada syndrome (BrS), including 
the presence of type 1 ST segment elevation and polymorphic ventricular 
tachycardia (PVT).  

The only gene thus far linked to BrS is SCN5A, the gene that encodes the alpha 
subunit of the cardiac sodium channel, which is named today BrS1 or first 
BrS. The cardiac sodium channel isoform encodes hH1 and has been mapped to 
the short arm of chromosome 3 p21-24 loci (Chen Q, Kirsch GE, Zhang D, 
Brugada R, Brugada P, Brugada J, et al. Genetic basis and molecular 
mechanisms for idiopathic ventricular fibrillation. Nature 1998; 
392:293-296). A second more benign form, known as BrS2, was mapped to 
chromosome 3p22-25. This variant was reported by Weiss et al (Weiss R, 
Barmada MM, Nguyen T, et al. Clinical and molecular heterogeneity in the 
Brugada syndrome: a novel gene locus on chromosome 3.Circulation. 2002; 
105:707-713) in a single large family. A BrS locus distinct from SCN5A is 
associated with progressive conduction disease, a low sensitivity to 
procainamide testing, and a relatively good prognosis. Screening of several 
candidates in the region (including two sodium channels, SCN510A and SCN512A) 
failed to identify the causal gene. 

ARVC/D Type 5: the affected locus was mapped to chromosome 3. The locus is 
3p21.3.3p23 and 3p25 deoxyribonucleic acid (DNA) haplotype at locus ARVD5 but 
different from Brugada Syndrome (BrS). Autosomal Dominant pattern of 
Inheritance.  Cytogenetics is 3p23 A peak 2-point lod score of 6.91, which 
was obtained with marker D3S3613 at a recombination fraction of 0.0. 
Haplotype analysis identified a shared region of 9.3 cM between markers 
D3S3610 and D3S3659. The gene symbols are ARVC5 or ARVD5 (Ahmad F, Li D, 
Karibe A, Gonzalez O, et al. Localization of a gene responsible for 
arrhythmogenic right ventricular dysplasia to chromosome 3p23. Circulation. 
1998;98: 2791-2795.). On the other hand, in the BrS the cardiac sodium 
channel isoform encodes hH1 and has been mapped to the short arm of 
chromosome 3 p21-24 loci.(Cheng Q, Kirsch GE, Zhang D, et al. Genetic basis 
and molecular mechanisms for idiopathic ventricular fibrillation. Nature. 
1998; 392:293-296).

Furthermore, the risk of SCD is probably variable among different types of 
ARVC/D. In Newfoundland, Canada, ARVD5 was reported to cause SCD in 44% of 
affected males, while females had a more benign course with no SCD. It is 
coincident with BrS (Dicks E, Hodgkinson K, Conners S. Initial clinical 
manifestations of arrhythmogenic right ventricular cardiomyopathy. Am J Hum 
Gen 2000; 67: 114a.).

The unknown mutation at the ARVD5 locus causing ARVC/D results in high 
mortality. Risk stratification using genetic haplotyping and ICD therapy 
produced improved survival for males. In the high risk group, 50% of males 
were dead by 39 years and females by 71 years: relative risk of death was 5.1 
(95% confidence interval 3 to 8.5) for males. The five-year mortality rate 
after ICD in males was zero compared with 28% in control subjects (p = 
0.009). Within five years, the ICD fired for VT in 70% and for VT >240 
beats/min in 30%, with no difference in discharge rate when analyzed by ICD 
indication. (Hodgkinson KA, Parfrey PS, Bassett AS, Kupprion C, Drenckhahn J, 
Norman MW, et al. The impact of implantable cardioverter-defibrillator 
therapy on survival in autosomal-dominant arrhythmogenic right ventricular 
cardiomyopathy (ARVD5). J Am Coll Cardiol. 2005; 45:400-408.). 

I think _different of others- , that the complications of Endomyocardial 
Biopsy are insignificant, and because of its important benefit for an 
accurate diagnosis, the procedure is recommend for differential diagnosis 
between both entities and it would mean an end in the speculations.(Benedek 
I, Hintea T.Endomyocardial biopsy in diagnosis of myocardial diseases.Rom J 
Intern Med. 1999;37:207-215.)

Best regard

Andrés Ricardo Pérez Riera

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Portugués

Em 1996, Corrado y col (Corrado D, Nava A, Buja G, et al. Familial 
cardiomyopathy underlies syndrome of right bundle branch block, ST segment 
elevation and sudden death. J Am Coll Cardiol 1996, 27: 443-448) aprestaram 
dados mostrando que existia uma sub-população de pacientes portadores da DAVD 
chamadas "formas ocultas" que se apresentavam com as características clínicas 
típicas da syndrome de Brugada incluído o padrão tipo 1 eletrocardiográfico e 
taquicardia ventricular polimórfica.  

O único gene identificado na SBr é o SCN5A o qual codifica a sub-unidade alfa 
do canal de sódio e se chama hoje SBr1 ou SBr primeiro. A isoforma do canal 
de sódio codifica hH1 ha sido mapeada no braço curto do cromossomo 3p21-24  
(Chen Q, Kirsch GE, Zhang D, Brugada R, Brugada P, Brugada J, et al. Genetic 
basis and molecular mechanisms for idiopathic ventricular fibrillation. 
Nature 1998; 392:293-296). Uma forma mais benigna conhecida como SBr2, foi 
mapeada no cromossomo 3p22-25. Este variante fora reportada por Weiss e col. 
Numa única família numerosa. (Weiss R, Barmada MM, Nguyen T, et al. Clinical 
and molecular heterogeneity in the Brugada syndrome: a novel gene locus on 
chromosome 3. Circulation. 2002; 105:707-713) Esta forma  BrS2 com locus 
diferente  do SCN5A se associa a doença de condução progressiva, baixa 
sensibilidade ao tese de procainamida e possui relativamente bom prognóstico. 
Rastreamento em vários candidates na região incluindo dos canais de sódio  
SCN510A e SCN512A não conseguiram identificar o gene causal. 

Na DAVC/D tipo 5 o lócus afetado tem sido mapeado no cromossomo 3 locus 
3p21.3.3p23 e 3p 25 no DNA haloptipo porém, diferente da SBr  A herença é 
autossômica dominante, a citogenetica é 3p23 Um pico2-punto lod escore de 
6.91, o qual fora obtido com marcador D3S313 na fração recombinante 0,0.  A 
análise do Halotipo identificou uma região compartilhada de 9.3 entre os 
marcadores DeS3610 e D3S3659. O símbolo do gene é ARVC5 ou ARVD5 (Ahmad F, Li 
D, Karibe A, Gonzalez O, et al. Localization of a gene responsible for 
arrhythmogenic right ventricular dysplasia to chromosome 3p23. Circulation. 
1998;98: 2791-2795.). Pelo contrário, na SBr a isoforma hH1 do canal de sódio 
ha sido mapeada no braço curto do cromossomo 3p21-24  locus. (Cheng Q, Kirsch 
GE, Zhang D, et al. Genetic basis and molecular mechanisms for idiopathic 
ventricular fibrillation. Nature. 1998; 392:293-296).

Além disso, o risco de SCD é provavelmente variável entre tipos diferentes de 
DAVD. Em Newfoudland Canadá A DAVD tipo 5 há sido reportada causando MCS em 
44% dos homens, ao passo que nas mulheres apresentam uma evolução mais 
benigna sem MCS fato coicidente com a SBr  (Dicks E, Hodgkinson K, Conners S. 
Initial clinical manifestations of arrhythmogenic right ventricular 
cardiomyopathy. Am J Hum Gen 2000; 67: 114a.).

Uma mutação desconhecida do tipo DAVD5 resulta em elevada taxa de mortalidade. 
A estratificação do risco utilizando a halotipificação e terapia com ICD 
ocasionou grande melhor do prognóstico no sexo masculino. No grupo de alto 
risco 50% dos homens faleceram aos 39 anos e as mulheres a os 71 anos. O 
risco relativo de morte foi de 5.1 (95% de intervalo de confidência e a 8.5) 
para homens.  A taxa de mortalidade apos o implante do ICD aos 5 anos foi de 
zero comparado com 28% nos controles ( p =0.009) . Dentro de 5 anos, o  ICD 
ateado fogo para TV em 70% e para TV 240 b/min em 30%, com nenhuma diferença 
na taxa da descarga quando analisado pela indicação de ICD. Hodgkinson KA, 
Parfrey PS, Bassett AS, Kupprion C, Drenckhahn J, Norman MW, et al. The 
impact of implantable cardioverter-defibrillator therapy on survival in 
autosomal-dominant arrhythmogenic right ventricular cardiomyopathy (ARVD5). J 
Am Coll Cardiol. 2005; 45:400-408.).

Eu penso que a complicações da biopsia endomiocárdica são insignificante 
diferentemente de outros e porque este daria um beneficio importante para um 
diagnóstico apurado o procedimento é recomendado para o diagnóstico 
diferencial  e determinação a existência ou não de um link entre ambas 
entidades..(Benedek I, Hintea T.Endomyocardial biopsy in diagnosis of 
myocardial diseases.Rom J Intern Med. 1999;37:207-215.)

Grato

Andrés Ricardo Pérez Riera

-- 
Dr. Sergio Dubner
Director

Dr. Edgardo Schapachnik
Director




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